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                Reagent Center

                Fast Lysis Method

                • BioFast Soil Genomic DNA Extraction Kit MORE
                  Product Details:

                  Kit Components

                  Cat#

                  BSC21S1

                  Components

                  50Tests

                  SP Buffer

                  45ml

                  Lysis S Buffer

                  5ml

                  DA Buffer

                  12.5ml

                  Binding Buffer

                  35ml

                  Wash Buffer

                  30ml(add 45ml absolute ethanol before use)

                  Elution Buffer

                  15ml

                  Grind Tube

                  50tubes

                  Spin Column

                  50tubes

                  Handbook

                  1copy

                  Introduction

                      The kit provides a very simple, fast and economic way for the isolation of PCR-ready genomic DNA from soil, adopting the Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields genomic DNA less than 30minutes. It does not require expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform.

                      At first, the soil sample is lysed by SP buffer and Lysis S Buffer in the grind tube. Designed for using with the lysis instruments from BIOER,soil is easily lysed within 40 s.Also manual operation can be chose with vortex generator within 5minutes.

                      Then DNA in the sample is liberated. After centrifuging, the impurity will be discarded. Released DNA is bound exclusively and specifically to the Biospin membrane in presence of a Binding Buffer under appropriate salt iron and pH conditions. Denatured protein and other contaminants are removed by several washing procedures. The DNA is then eluted from the membrane with the Elution Buffer.

                  Product Features:
                  Storage

                  All reagents, when stored properly, are stable for 18 months.

                  Apparatus and Materials to Be Supplied by the User

                  *  Sterile 2.0ml microcentrifuge tubes               * 10µl/100µl/1000µl tips

                  *  Microcentrifuge capable of 14,000g              * Absolute ethanol

                  Important notes

                  • 1. Please add 45ml absolute ethanol to Wash Buffer and mix thoroughly before the first use.
                  • 2. Lysis S Buffer may form precipitates upon storage. In case of precipitate forming, please incubate the buffer at 37°C until the precipitate has fully dissolved.
                  Experimental data:

                  Analysis DNA

                  • Absorbance analysis

                  Get some DNA,diluted in a advisable factor with elution buffer. Survey the OD260,OD280 and OD320.

                  Expressions:concentration(μg/ml)=50×OD260×dilution fact

                  Target: 2.0≥OD260-320/ OD280-320≥1.7

                  Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

                  • Agarose Gel Analysis

                  0.8~1% Agarose gel

                  Real-time Taqman PCR detect


                  Manual download
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